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The purification of ammonia monooxygenase from Paracoccus denitrficans

J. Moir,Lisa C. Crossman,S. Spiro,D. Richardson

1996 · DOI: 10.1016/0014-5793(96)00463-2
FEBS Letters · 125 Citations

TLDR

The heterotrophic nitrifier Paracoccus denitrificans expresses a membrane‐associated ammonia monooxygenase that has been solubilized in the detergent dodecyl‐β‐d‐maltoside and purified by standard chromatographic techniques, which is the first purification of an ammonia mono Oxygenase.