Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus
Structure of sortase, the transpeptidase that anchors proteins to the cell wall of Staphylococcus aureus
U. Ilangovan,H. Ton-That,2 Authors,R. Clubb
2001 · DOI: 10.1073/pnas.101064198
Proceedings of the National Academy of Sciences of the United States of America · 315 Citations
TLDR
The NMR structure of sortase reveals a unique β-barrel structure, in which the active-site sulfhydryl of cysteine-184 is poised for ionization by histidine-120, presumably enabling the resultant thiolate to attack the LPXTG peptide.