Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
Spatial-CITE-seq: spatially resolved high-plex protein and whole transcriptome co-mapping
Yang Liu,Marcello DiStasio,12 Authors,R. Fan
TLDR
Spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site.
Abstract
We present spatial-CITE-seq for high-plex protein and whole transcriptome co-mapping, which was firstly demonstrated for profiling 189 proteins and transcriptome in multiple mouse tissue types. It was then applied to human tissues to measure 273 proteins and transcriptome that revealed spatially distinct germinal center reaction in tonsil and early immune activation in skin at the COVID-19 mRNA vaccine injection site. Spatial-CITE-seq may find a range of applications in biomedical research.