Revolutionizing tissue regeneration: the art of mimicking diverse collagen-based extracellular matrix hierarchies with decellularized tendon via bioskiving.

Precisely simulating the collagen-based extracellular matrix (ECM) hierarchy is essential for advancing tissue regeneration. Although decellularized ECM (dECM) demonstrates considerable potential in fostering tissue regeneration by maintaining the inherent hierarchy, its application remains restricted to homologous tissues due to hierarchical variability. The current challenge involves overcoming obstacles that impede the application of dECM across diverse tissues, with particular emphasis on re-engineering one type of dECM to mimic the complex hierarchies of other tissues. The decellularized tendon, distinguished by its aligned collagen fibers, has garnered substantial interest due to its favorable structural and mechanical properties. Recently, a novel technique termed bioskiving has been developed, which uniquely disassembles macroscale decellularized tendons into microscale collagen sheets via microtome sectioning, thereby preserving micro/nano architecture of fiber alignment without chemical denaturation. These sheets can be further rotationally stacked or rolled to engineer 2D or 3D constructs replicating diverse ECM hierarchies. Bioskiving effectively dismantles the persistent hindrance that traditionally impeded the application of dECM across various tissues. This review offers an in-depth analysis of the fabrication procedure and characterization of constructs produced through bioskiving. It specifically elucidates the applications of constructs across various domains in tissue engineering, demonstrating its potential in overcoming tissue-specific constraints of dECM.