Quantitation of non-derivatized free amino acids for detecting inborn errors of metabolism by incorporating mixed-mode chromatography with tandem mass spectrometry
Quantitation of non-derivatized free amino acids for detecting inborn errors of metabolism by incorporating mixed-mode chromatography with tandem mass spectrometry
P. DeArmond,Dustin R. Bunch
2022 · DOI: 10.1016/j.jmsacl.2022.05.002
Journal of Mass Spectrometry and Advances in the Clinical Lab · 8 Citations
TLDR
Analysis of 39 free amino acids in 15 min LC-MS/MS run Chromatographically resolves leucine, isoleucines, and allo-isoleucine and the method validates in both plasma and urine.
Abstract
Highlights • Analysis of 39 free amino acids in 15 min LC-MS/MS run.• Validation of method in both plasma and urine.• Chromatographically resolves leucine, isoleucine, and allo-isoleucine.