Pilot Scale Bioethanol Production from Sugar Cane Bagasse through Separate Hydrolysis Fermentation by Saccharomyces cerevisiae

Bioethanol was produced at pilot scale from sugarcane bagasse by using Saccharomyces cerevisiae strain. The maximum obtained yield was 0.31 gp/gs after the fermentation of hydrolyzates by using microbial fermenter having capacity 30 L under the controlled pH 5.5±0.2 and Temperature 35°C. The conversion of lignocellulosic biomass to bioethanol requires pretreatment of biomass, Enzymatic Hydrolysis and Fermentation process. Both alkaline and acidic pretreatment techniques (NaOH+H2O2) with different compositions were used in this study. The Enzymatic hydrolysis was performed by using commercial cellulase enzyme and 55% hydrolysis rate has been achieved. The amount of ethanol increases from 9 % to 29 % through the process of simple distillation. The amount of ethanol further increases from 29 % to 95 % through the process of distillation using fractional distillation column. Gas Chromatography is an analytical technique used for the estimation of ethanol in purification process