NEPHROPROTECTIVE EFFECT OF CELASTRUS PANICULATUS SEED EXTRACT AGAINST LEAD ACETATE INDUCED TOXICITY IN WISTAR RATS

Background:Understanding of chemical induced nephrotoxicity is essential to prevent kidney disease including acuteand chronic kidney disease. Lead acetate is a toxic substance, andits clinically proven to cause renal toxicity. Nephrotoxicity caused by lead acetate is acomplex phenomenon, the importantcharactersitics are increase inserum urea and creatinine levels with significant change in the body weight. Many Indian herbs show ameliorating effects against heavy metal such as lead, mercury, and arsenic induced kidney damage. Use of these herbs against nephrotoxicity has gained momentum in recent times. Hence this study aimed to assess the effect of Celastrus paniculatus seeds against lead acetate induced nephrotoxicity in Wistar rats. Materials and Methods: The animals were divided into five groups, each group with six animals and were treated as follows: Group I, control; distilled water 2mL/kg b.w/day/orally, Group. II, Lead acetate (PbA) 30mg/kg b.w/day/orally; Group.III, PbA + N-acetylcysteine (NAC) 200mg/kg b.w/day/orally; Group. IV & V, PbA + Celastrus paniculatus in two different doses of 400mg and 800mg/kg b.w/day/orally for 28 days were administrated. Result:Lead acetate significantly decreases the serum urea and creatinine levels.Celastrus paniculatus seeds showed protective effects at 400 and 800mg/kg b.w by significantly reducing the levels of serum urea and creatinine as compared to the lead acetate administrated animals. The data were analyzed with SIGMA PLOT 13 using SYSTAT software and it was expressed as mean+ SE and the values with P < 0.05 were considered statistically significant. Conclusion:To conclude, the results suggest that ethanolic extract Celastrus paniculatus seedexhibits nephroprotective effect against lead acetate induced renal damage and in future, its mechanism of action are validated.