Metagenomics uncovers gaps in amplicon-based detection of microbial diversity
Metagenomics uncovers gaps in amplicon-based detection of microbial diversity
E. Eloe-Fadrosh,Natalia N. Ivanova,T. Woyke,N. Kyrpides
2016 · DOI: 10.1038/nmicrobiol.2015.32
Nature Microbiology · 200 Citations
TLDR
It is shown that approximately 10% of environmental microbial sequences might be missed from classical PCR-based SSU rRNA gene surveys, mostly members of the Candidate Phyla Radiation (CPR) and as yet uncharacterized Archaea.