Measuring the elastic modulus of soft culture surfaces and three-dimensional hydrogels using atomic force microscopy
Measuring the elastic modulus of soft culture surfaces and three-dimensional hydrogels using atomic force microscopy
Michael D A Norman,S. A. Ferreira,2 Authors,E. Gentleman
2021 · DOI: 10.1038/s41596-021-00495-4
Nature Protocols · 126 Citations
TLDR
A protocol for measuring E of soft 2D surfaces and 3D hydrogels using atomic force microscopy (AFM) force spectroscopy using either the Hertz or the Oliver-Pharr model to calculate E and instructions for sample preparation, instrument calibration and data collection and analysis are provided.