Evaluation of strategies for overcoming trifluoroacetic acid ionization suppression resulted in single-column intact level, middle-up, and bottom-up reversed-phase LC-MS analyses of antibody biopharmaceuticals.
Evaluation of strategies for overcoming trifluoroacetic acid ionization suppression resulted in single-column intact level, middle-up, and bottom-up reversed-phase LC-MS analyses of antibody biopharmaceuticals.
M. Khalikova,Laimonas Skarbalius,3 Authors,J. Lenčo
2021 · DOI: 10.1016/j.talanta.2021.122512
Talanta: The International Journal of Pure and Applied Analytical Chemistry · 19 Citations
TLDR
This mobile phase combined with the BioResolve RP mAb Polyphenyl column was subsequently demonstrated to provide excellent results for peptide mapping of antibody biopharmaceuticals fully comparable with those obtained using a state-of-the-art column for collagen separation, thus opening an avenue for a single-column multilevel analysis of these biotherapeutics.