Elongation factor G initiates translocation through a power stroke
Elongation factor G initiates translocation through a power stroke
Chunlai Chen,Xiaonan Cui,4 Authors,Y. Goldman
2016 · DOI: 10.1073/pnas.1602668113
Proceedings of the National Academy of Sciences of the United States of America · 58 Citations
TLDR
The rotational motions of EF-G domains during normal translocation detected by single-molecule polarized total internal reflection fluorescence (polTIRF) microscopy demonstrate that the ribosome andEF-G make use of power-stroke and Brownian-ratchet mechanisms to ensure efficiency and accuracy of translocation.