Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning.
Efficient generation of monoclonal antibodies from single human B cells by single cell RT-PCR and expression vector cloning.
T. Tiller,E. Meffre,3 Authors,H. Wardemann
2008 · DOI: 10.1016/J.JIM.2007.09.017
JIM - Journal of Immunological Methods · 1,117 Citations
TLDR
The method to clone and express human monoclonal antibodies is unbiased, highly efficient, requires only small cell numbers and the recombinant antibodies allow direct conclusions on the frequency of specific human B cells in a diverse repertoire.