DPS - a rapid method for genome sequencing of DNA-containing bacteriophages directly from a single plaque.
DPS - a rapid method for genome sequencing of DNA-containing bacteriophages directly from a single plaque.
W. Kot,F. Vogensen,S. Sørensen,L. Hansen
2014 · DOI: 10.1016/j.jviromet.2013.10.040
Journal of Virological Methods · 51 Citations
TLDR
A combination of host-DNA removal followed by purification and concentration of the viral DNA, allowed the construction of Illumina-compatible sequencing libraries using the Nextera XT technology directly from single phage plaques without any whole genome amplification step.