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DNA extraction protocols may influence biodiversity detected in the intestinal microbiome: a case study from wild Prussian carp, Carassius gibelio

E. Kashinskaya,K. Andree,E. Simonov,M. Solovyev

2017 · DOI: 10.1093/femsec/fiw240
FEMS Microbiology Ecology · 26 Citations

TLDR

It is shown that significant differences were observed in numbers of reads, OTUs, Shannon index and taxonomic composition between two different DNA extraction protocols for intestine of Prussian carp, and differences were also evident between microbial communities in the intestinal mucosa and intestinal content.