Dissecting virulence: systematic and functional analyses of a pathogenicity island.
Dissecting virulence: systematic and functional analyses of a pathogenicity island.
W. Deng,J. Puente,12 Authors,B. Finlay
2004 · DOI: 10.1073/PNAS.0400326101
Proceedings of the National Academy of Sciences of the United States of America · 650 Citations
TLDR
This work systematically mutagenized all 41 CR LEE genes and functionally characterized these mutants in vitro and in a murine infection model, identifying 33 virulence factors, including two virulence regulators and a hierarchical switch for type III secretion.