Coupling CRISPR scanning with targeted chromatin accessibility profiling using a double-stranded DNA deaminase.
Coupling CRISPR scanning with targeted chromatin accessibility profiling using a double-stranded DNA deaminase.
Heejin Roh,Simon P. Shen,10 Authors,Brian B Liau
2025 · DOI: 10.1038/s41592-025-02811-2
Nature Methods · 0 Citations
TLDR
TDAC-seq enables high-resolution sequence-function mapping of single-molecule chromatin fibers by genome editing and can be integrated with CRISPR perturbations to link genetic edits and their effects on chromatin accessibility on the same single chromatin fiber at single-nucleotide resolution.