COMPUTATIONAL ANALYSIS OF FUNCTIONAL EFFECTS OF SINGLE NUCLEOTIDE POLYMORPHISM ON OVINE INTERFERON TAU

The objective of this study was to evaluate the functional effects of Single Nucleotide Polymorphism(SNP) in ovine interferon tau using computational methods. Data on ovine interferon was retrieved from data base of National Center for Biotechnology Information ((GenBank). Functional effects of substitution of Leucin by Proline at position 26 of interferon protein chain  was predicted using  Protein Variation Effect Analyser, Panther server and Poly phen 2 (Polymorphism phenotyping). Provean score of  - 5.828 was obtained, indicating  a deleterious effect of the amino acid substitution. Panther server indicated damaging effect of the  SNP with Pdel ( probability of deleterious effect) of 0.5.  Similarly, Poly phen 2  showed damaging effect of the amino acid substitution. It can be concluded that substitution of Leucin  by proline at position 26 of  the protein sequence of interferon tau resulted in deleterious effect on the functionality of  ovine interferon tau. Studies in Single Nucleotide Polymorphism on ovine interferon tau can provide better understanding of biomarkers  associated with efficiency  of maternal pregnancy recognition in the ovine species.