Comparison of human IgG glycopeptides separation using mixed-mode hydrophilic interaction/ion-exchange liquid chromatography and reversed-phase mode

All ACE HILIC columns showed higher separation power for different glycoforms, and it is shown that each column separates a different group of glycopeptides more effectively than the others, and the possible retention mechanism for the HIL IC columns is discussed on the basis of the determined chromatographic parameters.