An improved method for western blotting when extracting proteins from mammalian cells cultured on a collagen gel under serum-free conditions
An improved method for western blotting when extracting proteins from mammalian cells cultured on a collagen gel under serum-free conditions
S. Ishihara,Takeomi Mizutani,K. Kawabata,H. Haga
2014 · DOI: 10.1007/s10616-014-9766-4
Cytotechnology (Dordrecht) · 8 Citations
TLDR
Improved western blotting method was able to detect p65 proteins without loss in A549 cells cultured on a collagen gel under serum-free conditions, but the proteins could not be detected if serum was present in cell culture.