An effective method for isolation of DNA from pig faeces and comparison of five different methods.
An effective method for isolation of DNA from pig faeces and comparison of five different methods.
Jun-ni Tang,Zhi-guang Zeng,10 Authors,Li-kou Zou
2008 · DOI: 10.1016/j.mimet.2008.07.014
Journal of Microbiological Methods · 60 Citations
TLDR
A new modified method for extracting PCR-quality microbial community DNA from pig faecal samples is described, which combines the pretreatment with polyformaldehyde, and subsequent DNA lysis in the presence of CTAB, salt, PVP, and beta-mercaptoethanol, followed by isolation of nucleic acids using chloroform (no phenol) based protocol.