A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.
A Sanger/pyrosequencing hybrid approach for the generation of high-quality draft assemblies of marine microbial genomes.
S. M. Goldberg,Justin Johnson,16 Authors,J. Venter
2006 · DOI: 10.1073/PNAS.0604351103
Proceedings of the National Academy of Sciences of the United States of America · 301 Citations
TLDR
This study evaluated the utility and cost-effectiveness of a hybrid sequencing approach using 3730xl Sanger data and 454 data to generate higher-quality lower-cost assemblies of microbial genomes compared to current Sanger sequencing strategies alone.