A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines.
J. Kearney,Andreas Radbruch,B. Liesegang,K. Rajewsky
TLDR
A subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains is isolated and can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies.
Abstract
We have isolated a subclone of the mouse myeloma cell line P3-X63-Ag8 that does not express immunoglobulin heavy or light chains. This clone X63-Ag8.653 can be used for efficient fusion with antibody-forming cells to obtain hybrid cell lines producing pure monoclonal antibodies. Screening of hybrid cell lines for specificity and immunoglobulin classes was done with a modified enzyme-linked immunosorbent assay.