A continuous spectrophotometric enzyme-coupled assay for deoxynucleoside triphosphate triphosphohydrolases.
A continuous spectrophotometric enzyme-coupled assay for deoxynucleoside triphosphate triphosphohydrolases.
D. Singh,R. Schaaper,A. Hochkoeppler
2016 · DOI: 10.1016/j.ab.2015.11.027
Analytical Biochemistry · 8 Citations
TLDR
The enzyme-coupled assay proved to be more sensitive than the chromatographic procedure, permitting reliable detection of Dgt activity at low dGTP substrate concentrations and with a discontinuous high-performance liquid chromatography method able to selectively detect deoxyguanosine.