Uv-visible spectroscopy of bacteriorhodopsin mutants: substitution of Arg-82, Asp-85, Tyr-185, and Asp-212 results in abnormal light-dark adaptation.
Uv-visible spectroscopy of bacteriorhodopsin mutants: substitution of Arg-82, Asp-85, Tyr-185, and Asp-212 results in abnormal light-dark adaptation.
M. Duñach,T. Marti,H. Khorana,K. Rothschild
1990 · DOI: 10.1073/PNAS.87.24.9873
Proceedings of the National Academy of Sciences of the United States of America · 42 Citations
TLDR
It is suggested that in all of these mutants, the chromophore has lost the ability to undergo the normal 13-cis,15-syn to all-trans, 15-anti light-driven isomerization, which occurs in native bR, except transiently.