Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease
Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease
V. Nekrasov,B. Staskawicz,2 Authors,S. Kamoun
2013 · DOI: 10.1038/nbt.2655
Nature Biotechnology · 1,029 Citations
TLDR
Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease is demonstrated using a single Cas9 molecule for the first time.