Oral Presentations - Abstracts of the 27th Annual Congress of the SBRA. Aracaju/SE - Brazil, 2023

Objective: To create metabolomics models capable of seg-regating men who improved semen analysis (SA) parameters after microsurgical varicocelectomy (MV) from those who did not, using Hydrogen-1 nuclear magnetic resonance (1H NMR) spectra of blood serum from of preoperative samples. Methods: We recruited 32 infertile men with palpable vari-cocele and abnormal semen analysis (SA). Preoperative peripheral venous blood samples were centrifuged at 3000 g for 15 minutes to separate the cellular component from the supernatant serum. The blood serum samples were kept frozen at –40°C until analysis. 1H NMR spectra of blood serum were obtained and used to create metabonomics models. All participants were treated with artery and lymphatic sparing MV by the same surgeon. Improvement was defined as an increase of at least 20% in the total motile progressive sperm count (TMC) of the post-operative SA when compared to the baseline. Results: Eighteen (56%) participants had SA improvement after MV and were included in the improved (I) group, the remaining 14 participants were included in the non-improved (NI) grop. There were no differences regarding the baseline characteristics and SA parameters. The I group had lower baseline luteinizing hormone levels when compared to the NI group (4.3 vs 7.0 mUI/mL), and