Optical Recording Reveals Novel Properties of GSK1016790A-Induced Vanilloid Transient Receptor Potential Channel TRPV4 Activity in Primary Human Endothelial Cells
Optical Recording Reveals Novel Properties of GSK1016790A-Induced Vanilloid Transient Receptor Potential Channel TRPV4 Activity in Primary Human Endothelial Cells
M. Sullivan,Michael Francis,2 Authors,S. Earley
2012 · DOI: 10.1124/mol.112.078584
Molecular Pharmacology · 61 Citations
TLDR
A novel, highly efficient method for recording and analyzing Ca2+-permeable channel activity in primary human endothelial cells using a unique combination of total internal reflection fluorescence microscopy (TIRFM), custom software-based detection, and selective pharmacology is described.