One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products.
K. Datsenko,B. Wanner
2000 · DOI: 10.1073/PNAS.120163297
Proceedings of the National Academy of Sciences of the United States of America · 15,610 Citations
TLDR
A simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s), which should be widely useful, especially in genome analysis of E. coli and other bacteria.