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New shuttle vectors for Escherichia coil and Bacillus subtilis. II. Plasmid pHY300PLK, a multipurpose cloning vector with a polylinker, derived from pHY460

H. Ishiwa,H. Shibahara,H. Shibahara

2006 · DOI: 10.1266/jjg.60.235
23 Citations

TLDR

A new cloning vector that can replicate and express the tetracycline resistance gene (TcR) in both Escherichia coli and Bacillus subtilis is constructed and characterized as a copy-number mutant in E. coli.