Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System
Yu Jiang,Biao Chen,3 Authors,Sheng Yang
2015 · DOI: 10.1128/AEM.04023-14
Applied and Environmental Microbiology · 1,042 Citations
TLDR
A targeted, continual multigene editing strategy that was applied to the Escherichia coli genome by using the Streptococcus pyogenes type II CRISPR-Cas9 system to realize a variety of precise genome modifications, including gene deletion and insertion, with the highest efficiency of 100%, is described.