Investigation the effects of green synthesized ZnO nanoparticles on the viability of L929 fibroblasts using MTT assay

Zinc oxide nanoparticles (ZnONPs) were prepared by eco-friendly method using the alcoholic extract of Ginger( Zingiber officinale) , this study conducted to evaluate the cytotoxic effect of Zinc oxide nanoparticles on viability of L929 fibroblast cell line for 72 hours and incubation with (1, 2, 4, 8, and 16 mg/ml) of GnZnO NPs , Several methods were used to characterize the biosynthesized GnZnONPs. including "UV-Visible Spectroscopy," Field Emission Scanning Electron Microscope ("FESEM"), " Infrared Spectroscopy (FTIR)" and X-Ray Diffraction (XRD) spectrum. the MTT assay was used to determine the cytotoxicity effect of Zinc oxide nanoparticles (ZnONPs) on the, cell line viability The results showed that there was no toxic effect of the synthesis GnZnONPs with an increase in concentration from (1-8) mg/ml after 72 hours of incubation at 37°C. The findings of this study, there was a time and dose-dependent reduction in cell viability in treated cell lines after exposure to GnZnO-NPs for 3 days, with no adverse effect on fibroblast cells till 8 mg/ml.