Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.
Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.
H. Shizuya,B. Birren,4 Authors,Melvin I. Simon
1992 · DOI: 10.1073/PNAS.89.18.8794
Proceedings of the National Academy of Sciences of the United States of America · 1,655 Citations
TLDR
Because of high cloning efficiency, easy manipulation of the cloned DNA, and stable maintenance of inserted DNA, the BAC system may facilitate construction of DNA libraries of complex genomes with fuller representation and subsequent rapid analysis of complex genomic structure.