Arabidopsis ORP2A mediates ER–autophagosomal membrane contact sites and regulates PI3P in plant autophagy
Arabidopsis ORP2A mediates ER–autophagosomal membrane contact sites and regulates PI3P in plant autophagy
Hao Ye,Jiayang Gao,4 Authors,Liwen Jiang
TLDR
A distinct molecular architecture of an endoplasmic reticulum–autophagosomal membrane contact site (EACS) in regulating plant autophagy is illustrated, which involves the lipid-binding protein ORP2A, the ER residential protein VAP27-1, and the ubiquitin-like protein ATG8e.
Abstract
Significance We illustrated a distinct molecular architecture of an endoplasmic reticulum (ER)–autophagosomal membrane contact site (EACS) in regulating plant autophagy, which involves the lipid-binding protein ORP2A, the ER residential protein VAP27-1, and the ubiquitin-like protein ATG8e. We further demonstrated that ORP2A functions in autophagosome formation, while knocking down of ORP2A affects phosphatidylinositol 3-phosphate (PI3P) redistribution.