Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining.
Analysis of Simian Immunodeficiency Virus-specific CD8+ T-cells in Rhesus Macaques by Peptide-MHC-I Tetramer Staining.
Lucas Gonzalez-Nieto,Aline Domingues,6 Authors,M. Martins
2016 · DOI: 10.3791/54881
Journal of Visualized Experiments · 14 Citations
TLDR
An optimized protocol for enumerating SIV-specific CD8+ T-cells in rhesus macaques by pMHC-I tetramer staining is presented, applicable for studying CD8- T-cell responses in multiple disease settings.