An evaluation of the double thymidine block for synchronizing mammalian cells at the G1-S border.
An evaluation of the double thymidine block for synchronizing mammalian cells at the G1-S border.
C. Bostock,David M. Prescott,J. Kirkpatrick
1971 · DOI: 10.1016/0014-4827(71)90599-4
Experimental Cell Research · 177 Citations
TLDR
The results indicate that cells continue to synthesize DNA during the thymidine block at about one-third the normal rate and do not accumulate at the G 1-S border.