A plasmid RK2-based broad-host-range cloning vector useful for transfer of metagenomic libraries to a variety of bacterial species.
A plasmid RK2-based broad-host-range cloning vector useful for transfer of metagenomic libraries to a variety of bacterial species.
Trine Aakvik,K. Degnes,6 Authors,S. Valla
2009 · DOI: 10.1111/j.1574-6968.2009.01639.x
FEMS Microbiology Letters · 76 Citations
TLDR
The construction of a broad-host-range plasmid vector (pRS44) for fosmid and bacterial artificial chromosome (BAC) cloning and no other reported vector system has a comparable potential for functional screening across species barriers is reported.