Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs.
Bioinspired negatively charged calcium phosphate nanocarriers for cardiac delivery of MicroRNAs.
V. Di Mauro,M. Iafisco,7 Auteurs,D. Catalucci
2016 · DOI: 10.2217/nnm.16.26
Nanomedicine · 86 citaten
TLDR
CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes and successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells in vitro and in vivo.
Samenvatting
AIM
To develop biocompatible and bioresorbable negatively charged calcium phosphate nanoparticles (CaP-NPs) as an innovative therapeutic system for the delivery of bioactive molecules to the heart.MATERIALS & METHODS
CaP-NPs were synthesized via a straightforward one-pot biomineralization-inspired protocol employing citrate as a stabilizing agent and regulator of crystal growth. CaP-NPs were administered to cardiac cells in vitro and effects of treatments were assessed. CaP-NPs were administered in vivo and delivery of microRNAs was evaluated.RESULTS
CaP-NPs efficiently internalized into cardiomyocytes without promoting toxicity or interfering with any functional properties. CaP-NPs successfully encapsulated synthetic microRNAs, which were efficiently delivered into cardiac cells in vitro and in vivo.CONCLUSION
CaP-NPs are a safe and efficient drug-delivery system for potential therapeutic treatments of polarized cells such as cardiomyocytes.