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Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.
K. Livak,Thomas D. Schmittgen
2001 · DOI: 10.1006/METH.2001.1262
Methods · 159,265 citations
TLDR
The 2(-Delta Delta C(T)) method is a convenient way to analyze the relative changes in gene expression from real-time quantitative PCR experiments and two variations of the method are presented that may be useful in the analysis of real- time, quantitative PCR data.