Multiplex Genome Engineering Using CRISPR/Cas Systems
Multiplex Genome Engineering Using CRISPR/Cas Systems
Le Cong,F. Ran,8 作者,Feng Zhang
2013 · DOI: 10.1126/science.1231143
Science · 引用数 14,166
TLDR
Two different type II CRISPR/Cas systems are engineered and it is demonstrated that Cas9 nucleases can be directed by short RNAs to induce precise cleavage at endogenous genomic loci in human and mouse cells, demonstrating easy programmability and wide applicability of the RNA-guided nuclease technology.